@article {1342, title = {Antioxidant and Cytotoxic Bioassay on Blumeodendron toxbrai (Blume.) Stem Bark Hexane, Dichloromethane, and Methanolic Ekstract}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {139-141}, type = {Research Article}, chapter = {139}, abstract = {

Introduction: Blumeodendron toksbraii has the potential to be anti-HIV and anti α-glucosidase. Objective: This research was conducted to examine the effects of antioxidant and cytotoxicity in vitro from these compounds from methanolic stem bark extract. Method: Stem bark to be extracted with maceration using hexane, dichloromethane, and methanol solution. Extracts were quantified with respect to in vitro antioxidant activity using the 2.2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging. Anticytotoxic activity was determined by cytotoxicity assay using MCF-7 cell line with Alamar Blue method. Results: The observed IC50 value from hexane, dichloromethane, and methanol extract for antioxidant assay were 88.33 {\textpm} 0.19 μg/ mL, 74,54 {\textpm} 0,61 μg /mL and 94.1 {\textpm} 0.19 μg/mL respectively. IC50 value of anti-cytotoxic assay from hexane extract, dichloromethane and methanol extract is 121.24 {\textpm} 0.15 μg/mL, 55 {\textpm} 0,48 μg/mL and 70.71 {\textpm} 0.15 μg/mL. Conclusion: dichloromethane extract showed good promising result for anti-oxidant and cytotoxic assay, futher study needed to isolate compound from this plant.

}, keywords = {Anticytotoxic DPPH, Antioxidant, Blumeodendron toksbraii, Cancer, MCF-7}, doi = {10.5530/pj.2021.13.19}, author = {Andreas Susilo Adi and Berna Elya and Muhammad Hanafi} }