Anti-migration and Anti-invasion Abilities of Methanolic Leaves Extract of Clerodendrum Inerme on Lung Cancer Cells

Background: Clerodendrum inerme is a folk medicinal plant used in the treatment of various illnesses such as a cough, scrofulous infection, venereal infection, skin diseases and tumors. It exhibited many pharmacological effects including hepatoprotective, anti-inflammatory, antibacterial, anti-oxidant and anticancer properties. Objective: The purpose of this study was to investigate the influence of methanolic extract of C. inerme leaves on migration, invasion and adhesion activities on human lung adenocarcinoma. Materials and methods: Cytotoxicity, cell motility, migration, invasion and adhesion abilities were detected by MTT, wound healing, trans-well mobilization, modified Boyden chamber and cell adhesion assay, respectively. Results: The results demonstrated that up to 400 μg/mL methanolic leaves extract has low toxicity on A549 cells showing more than 50% cell viability. At non-cytotoxic and sub-toxic doses (200 and 400 μg/mL) of methanolic leave extract significantly suppressed cell motility, migration, invasion and cell adhesion compared with the untreated control. Conclusion: These results suggested that methanolic leaves extract of C. inerme inhibited migration, invasion and adhesion of A549 cells. These findings showed new therapeutic potential for C. inerme in anti-metastatic therapy.