ArticleViewAbstractPharmacognosy Journal,2014,6,4,59-67.Published:26th May 2014Type:Research ArticleProtective effect of cissampelos pareira linn. Extract on gentamicin-induced nephrotoxicity and oxidative damage in ratsD. R Ch Sekhara Reddy, G Siva Kumar, B. M. Vrushabendra Swamy, and K. Phani Kumar D. R Ch Sekhara Reddy1*, G. Siva Kumar 2, B. M. Vrushabendra Swamy2 and K. Phani Kumar11Acharya Nagarjuna University College of Pharmaceutical Sciences, Nagarjuna Nagar, Guntur-522510, India2Gautham College of Pharmacy, Bhuvaneswari Nagar, Bengaluru-560032, India.Abstract:Objective: To evaluate the potential nephroprotective and antioxidant activity of hydroalcoholic Cissampelos pareira (C. pareira) whole plant extract using gentamicin-induced rats. Methods: For studying acute toxicity study, single oral dose of 2g/kg hydroalcoholic extract of C. pareira was evaluated in rats by oral gavage. The nephroprotective activity was evaluated using gentamicin-induced model in rats. In-vitro antioxidant activity was evaluated by using DPPH assay and reducing power assay. In-vivo antioxidant activity was evaluated by using glutathione and lipid peroxidation estimations in gentamicin-induced rats. Hydroalcoholic C. pareira whole plant extract was given at a dose of 200 and 400 mg/kg p.o. Results: For acute toxicity testing rats administered with the extract at a dose 2 g/ kg. the result showed no toxicity. Hydroalcoholic C. Pareira whole plant extract (200 and 400 mg/kg p.o) significantly decrease the elevated urinary glucose levels in the urine, decrease the elevated urea and creatinine levels in blood and increase the urinary creatinine levels in gentamicin-induced nephrotoxic rats. The extract had shown significant dose dependent increase in the DPPH and reducing power activity. There were a dose dependent decreasing and increasing of lipid peroxidation, glutathione levels in hydoalcoholic extract treated groups respectively. Conclusion: This study exhibits that hydroalcoholic C. pareira whole plant extract poses nephroprotective activity which may be due to its antioxidant activity.Key words: Gentamicin, Cissampelos pareira, creatinine, urea, glucose, lipid peroxidation, glutathione, reducing power, DPPH.Keywords:Cissampelos pareira, Creatinine, DPPH, Gentamicin, Glucose, Glutathione, Lipid peroxidation, Reducing power, urea