ArticleViewAbstractPharmacognosy Journal,2017,9,5,607-610.DOI:10.5530/pj.2017.5.96Published:July 2017Type:Original ArticleAntioxidant Activity and Isolation of Xanthine Oxidase Inhibitor from Ruellia tuberosa L. LeavesAktsar Roskiana Ahmad, Berna Elya, and Abdul Mun’im Aktsar Roskiana Ahmad1,2, Berna Elya1, Abdul Mun’im1* 1Department of Pharmacognosy- Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok 16424 West Java, INDONESIA 2Faculty of Pharmacy, Indonesia Muslim University, Makassar, INDONESIAAbstract:Introduction: The leaves of Ruellia tuberosa L. have been known to demonstrate strong antioxidant and xanthine oxidase (XOD) inhibitory activities. The aim of this study was to isolate antioxidant and XOD inhibitor from the leaves of the plants. Methods: Isolation of antioxidant and XOD inhibitor were conducted using chromatography techniques. The structure of the isolated compound was elucidated by spectroscopic methods. Results: In this study, a flavonoid was isolated and characterized as methoxylated flavonoid based on the spectral data including UV, IR, GC-MS, and NMR. The compound demonstrated DPPH free radical scavenging activity with IC50 of 28.79 μg/ml, and XOD inhibitory with IC50 of 0.67 μg/mL. Conclusion: The isolated compound was determined as 5-hydroxy-3,7-dimethoxy-2-(4-((3S,4S,5S,6R)-4,5, 6-trihydroxy-2(hydroxymethoxy)-tetrahydro-2H-pyrane-3-iloxy) phenyl)-4H-chromen-4-on or camarosids. The isolated compound demonstrated strong DPPH free radical scavenging and XOD inhibitory activity. Keywords:Antioxidant, DPPH, Flavonoid., Ruellia Tuberosa, Xanthine OxidaseView:PDF (737.07 KB) Full Text PDF Images Graphical Abstract ‹ Evaluation of Antidiabetic Activity of Hydroalcoholic Extract of Cassia fistula Linn. pod in Streptozotocin-Induced Diabetic Rats up Antibacterial and Antioxidant Activity of Black Mulberry (Morus nigra L.) Extract for Acne Treatment ›